The ANK61 monoclonal antibody was generated by immunizing mice with IL2-activated 
  cultured NK cells of Wag rats  (Giezeman-Smits et al., 1997).

  ANK61 reactivity is rat strain-independent.

  The antigen recognized by ANK61 is highly expressed on freshly isolated and cultured rat 
  NK cells. The antibodies also bind to rat aß-TCR  T cells and at a low level to rat  B cells. 
  Binding to other cell types is unknown. Triggering of  the antigen by  ANK61 antibodies 
  activates the lytic machinery of rat NK cells, but not of T cells.

  The ANK61 monoclonal antibody can be used in flow cytometry and for immunoprecipitation 
  of the antigen. It can also be used for immunohistochemical analysis sections of freshly frozen 
  tissues or formalin-fixed/paraffin-embedded tissues. Citric acid pretreatment is recommended 
  for the latter type of tissues.

  Giezeman-Smits KM, Gorter A, Nagelkerke JF, Van Vlierberghe RL, van Eendenburg J, 
  Eggermont AM, Fleuren GJ,  Kuppen PJ (1997) Characterization of three new membrane 
  structures on rat NK cells which are involved in activation of the  lytic machinery.  
  Immunobiology 197: 429-443