Monoclonal antibodies directed against rat CD45

 Hybridoma Clone    Antibody Isotype    Cat. No.
ANK74                      Mouse IgG1           ARA05
ANK12                      Mouse IgG1           ARA12

Generation
  The ANK74  and ANK12 monoclonal antibodies were generated  by immunizing 
  mice with IL2-activated cultured NK cells of Wag rats.
Reactivity 
  These are rat strain-independent antibodies to CD45.

  ANK74
  This monoclonal antibody recognizes a common epitope of rat CD45, present on all rat 
  leucocytes. 

  ANK12
  This monoclonal antibody is directed against an isoform of rat CD45.
Technical information 
  ANK74 and ANK12 bind to rat CD45. This antigen of approximately 200 kD is expressed on the 
  cell surface of leucocytes.  ANK74 and ANK12 can be used for the detection of leucocytes 
  by immunohistochemistry in  freshly frozen tissues or by flow cytometry. Both antibodies are 
  able to modulate lysis of target  cells by NK cells (Giezeman-Smits et al., 1999).
References 
  Giezeman-Smits KM, Gorter A, Van Vlierberghe RL, Eendenburg JD, Eggermont AM, Fleuren 
  GJ, Kuppen PJ (1999) The regulatory role of CD45 on rat NK cells in target cell lysis. 
  J Immunol 163: 71-76

Ordering Information