Monoclonal antibodies directed against rat tumor antigens of epithelial origin


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                "Binding of MG2 monoclonal antibodies to rat colorectal
                metastases in freshly frozen rat liver tissue"
Hybridoma Clone    Antibody Isotype    Cat. No.
MG1                           Mouse IgG2a        ARA02
MG2                           Mouse IgG2a        ARA10

Generation
  MG1 and MG2 have been raised by immunization 
  of mice with rat colon adenocarcinoma cells (CC531). Hybridomas were selected for 
  producing antibodies reacting with the CC531 cell line and with minimal cross reactivity 
  to normal tissues (Hagenaars et al., 2001). MG1 and MG2 belong to a second generation 
  of antibodies directed against tumors of epithelial origin that were strictly selected for 
  minimal cross reactivity with other tissues.
Reactivity 
  MG1 and MG2 are rat strain-independent markers for tumor cells of epithelial origin, like in 
  colon, breast, or lung cancer, etc. When injected in colon tumor-bearing rats, MG1 
  and MG2 localized to tumor cells (Hagenaars et al., 2001). MG1 and MG2 can be used 
  for immunohistochemical staining of tumor cells in frozen tissue sections as well as 
  formalin-fixed/paraffin-embedded tissue. MG1 and MG2  are also  suitable to be used 
  in flow cytometry, Western blotting, immunoprecipitation, and ELISA.
Technical information 
  MG1
  This monoclonal antibody recognizes a cell surface structure of about 80 kD expressed 
  by rat tumor cells of epithelial origin.  It is useful for in vivo applications as it has no detectable 
  binding to other cell types then tumor cells when injected in rats. MG1 is also useful for 
  immunohistochemical detection of tumor cells. In immunohistochemical stainings, there 
  is some background in thymus and lymph nodes. Citric acid or EDTA pretreatment is 
  recommended for formalin-fixed/paraffin-embedded tissue sections. 

  MG2
  This monoclonal antibody has the same characteristics as MG1.
References 
  Hagenaars M, Ensink NG, Basse P, Hokland M, Nannmark U, Eggermont AM, 
  Van De Velde CJ, Fleuren GJ, Kuppen PJ (2000) The microscopic anatomy of experimental 
  rat CC531 colon tumour metastases: consequences for immunotherapy? Clin Exp Metastasis 
  18: 189-196

  Hagenaars M, Ensink NG, Van Eendenburg JD, Van Vlierberghe RL, Eggermont AM, 
  Van De Velde CJ, Fleuren GJ, Kuppen PJ (2001) The development of novel mouse 
  monoclonal antibodies against the CC531 rat colon adenocarcinoma. Clin Exp Metastasis, 
  18: 281-289

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