MG3 has been raised by immunization of mice with rat colon 
  adenocarcinoma cells (CC531). Hybridomas were selected for producing antibodies 
  reacting with the CC531 cell line and with minimal cross reactivity to normal tissues 
  (Hagenaars et al., 2001). MG3 belongs to a second generation of such antibodies that 
  were strictly selected for minimal cross reactivity with other tissues

  MG3 is a rat strain-dependent marker for tumor cells of epithelial origin, like in colon, 
  breast, or lung  cancer, etc. When injected in colon tumor-bearing rats, MG3 did not 
  localize to tumor cells (Hagenaars et al., 2001). MG3 is able to activate the rat 
  complement system.  MG3 can be used for immunohistochemical staining of tumor cells 
  in frozen tissue sections as well as formalin-fixed/paraffin-embedded tissue. MG3 is also  
  suitable to be used in flow cytometry, Western blotting, immunoprecipitation, and ELISA.

  The antigen detected by this monoclonal antibody has not been characterized. The antigen
  is expressed  on the cell surface of rat tumor cells of epithelial origin. When bound to tumor 
  cells the antibody activates the rat complement system.

  Hagenaars M, Ensink NG, Basse P, Hokland M, Nannmark U, Eggermont AM, Van De Velde CJ, 
  Fleuren GJ, Kuppen PJ (2000) The microscopic anatomy of experimental rat CC531 colon 
  tumour metastases: consequences for immunotherapy? Clin Exp Metastasis 18: 189-196

  Hagenaars M, Ensink NG, Van Eendenburg JD, Van Vlierberghe RL, Eggermont AM, 
  Van De Velde CJ, Fleuren GJ, Kuppen PJ (2001) The development of novel mouse 
  monoclonal antibodies against the CC531 rat colon adenocarcinoma. Clin Exp Metastasis, 
  18: 281-289