Monoclonal antibodies directed against rat tumor antigens of epithelial origin
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                "Binding of MG4 (IgG1) monoclonal antibodies to rat colorectal
                metastases in freshly frozen rat liver tissue"
Hybridoma Clone    Antibody Isotype    Cat. No.
MG4                           Mouse IgG1           ARA07
MG4-2a                     Mouse IgG2a         ARA08
MG4-2b                     Mouse IgG2b         ARA09

Generation
  MG4 has been raised by immunization of mice with 
  rat colon adenocarcinoma cells (CC531). Hybridomas were selected for producing 
  antibodies reacting with the CC531 cell line and with minimal cross reactivity to normal 
  tissues (Hagenaars et al., 2001). MG4 belongs to a second generation of such antibodies 
  that were strictly selected for minimal cross reactivity with other tissues. In addition to the 
  original IgG1 isotype, IgG2a and IgG2b isotype switch variants were derived from MG4.
Reactivity 
  MG4 is a rat strain-independent marker for tumor cells of epithelial origin, like in colon, 
  breast, or lung cancer, etc. When injected in colon tumor-bearing rats, MG4 localized to 
  tumor cells (Hagenaars et al., 2001). MG4 can be used for immunohistochemical staining 
  of tumor cells in frozen tissue sections as well as formalin-fixed/paraffin-embedded tissue. 
  MG4 is suitable to be used in flow cytometry, Western blotting, immunoprecipitation, and ELISA.
Technical information 
  These monoclonal antibodies bind to two proteins, 120 kD and 130 kD, expressed by rat 
  tumor cells of epithelial origin. MG4 and the isotype switch variants are especially useful for 
  the detection of tumor cells by flow cytometry as the result is a relatively high signal and for 
  multicolor stainings.  MG4 can also be used in immunohistochemistry in both formalin-
  fixed/paraffin-embedded tissues and freshly frozen tissues, but the staining is less intense 
  as compared with CC52, MG1 or MG2. Citric acid pretreatment is recommended for formalin-
  fixed/paraffin-embedded tissue sections. MG4 can be used in western blotting.
References 
  Hagenaars M, Ensink NG, Basse P, Hokland M, Nannmark U, Eggermont AM, Van De Velde 
  CJ, Fleuren GJ, Kuppen PJ (2000) The microscopic anatomy of experimental rat CC531 colon 
  tumour metastases: consequences for immunotherapy? Clin Exp Metastasis 18: 189-196

  Hagenaars M, Ensink NG, Van Eendenburg JD, Van Vlierberghe RL, Eggermont AM, 
  Van De Velde CJ, Fleuren GJ, Kuppen PJ (2001) The development of novel mouse 
  monoclonal antibodies against the CC531 rat colon adenocarcinoma. Clin Exp Metastasis, 
  18: 281-289

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