Anti-human cytokeratin no. 13 (clone 1C7)
"Binding of 1C7 monoclonal antibodies to human cytokeratin no. 13"
Hybridoma Clone Antibody Isotype Cat. No.
Clone 1C7 Mouse IgG2a ARA23
Source
A mouse was immunized with cytokeratin purified from human esophagus. Antibody producing cells
were isolated after fusing the spleen cells of this mouse with murine myeloma cells by selecting the
cells that produced antibodies capable of staining human epithelia in thin sections.
Specification:
The antibody does not stain non-cornified squamous epithelia, except cornea and transitional epithelial
regions, with the exception of basal cell layers of some stratified epithelia (ref. 1). In immunoblotting
experiments the antibody was shown to recognize only cytokeratin no. 13 (54 kDa).
| | Immunohistochemistry (frozen sections) | yes |
| | Immunohistochemistry (formalin embedded tissue) | no |
| | Immunohistochemistry (antigen retrieval) | yes |
| | Flow cytometry | not tested |
| | Immunoblotting | yes |
| | ELISA | not tested |
| | RIA | not tested |
| | Cross reactivity |
| | Other cytokeratins | not detectable |
| | Vimentin | not detectable |
| | Desmin | not detectable |
| | Glial fibrillar acidic protein | not detectable |
| | Neurofilament | not detectable |
| | Mouse cytokeratin 13 | not tested |
| | Rat cytokeratin 13 | not tested |
| | Rabbit cytokeratin 13 | not tested |
| | Goat cytokeratin 13 | not tested |
Immunoglobulin (sub)class: IgG2a
Presentation: supernatant or protein A purified
Storage:
4°C = 2 months
-20°C = 1 year
Aliquoted storage at -70°C or –20°C is recommended
Reference:
1. Muijen GNP et al. (1986) Exp Cell Res 162, 97-113.
2. Muijen GNP et al. (1987) Exp Cell Res 171, 331-345.
3. Dorst EBL et al. (1998) J Clin Pathol 51, 679-684.
4. Corver WE et al. (1994) Cytometry 15, 117-128.
Ordering Information
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