Monoclonal antibodies directed against tissue inhibitor of matrix metalloproteinase-1 (TIMP-1)

Hybridoma Clone    Antibody Isotype    Cat. No
2A5                        Mouse IgG1          ARA33

Generation
The 2A5 monoclonal antibody was generated from mice immunized with a 11 mer-peptide derived from the sequence of human TIMP-1. The amino acid sequence of this peptide was: FQALGDAADIR (Murray et al., 1998).

Reactivity
This monoclonal antibody binds to human TIMP-1. Reactivity in other species has not been determined. The 11-mer peptide used for the immunization differs on at least 3 amino acids with the sequence of TIMP-1 of rat, mouse, and rabbit. Therefore it is not expected to be effective in these species as well. The antibody was tested for cross-reactivity with TIMP-2 and did not cross-react.

Technical information
The 2A5 monoclonal antibody has been tested for use in Western blotting and immunohistochemical analysis of formalin-fixed/paraffin-embedded tissues. It is suggested to use colon carcinoma as positive control. Antigen retrieval by microwave treatment (5-20 min at full power, 950 W in 0.01 M sodium citrate buffer, pH 6.0) is recommended for immunohistochemical analysis.

References
1. Murray GI, Duncan ME, Arbuckle E, Melvin WT, Fothergill JE (1998) Matrix metalloproteinases and their inhibitors in gastric cancer. Gut 43: 791-797