Monoclonal antibodies directed against matrix metalloproteinase-9 (MMP-9)

Hybridoma Clone    Antibody Isotype    Cat. No
2C3                      Mouse IgG1             ARA32

Generation
The 2C3 monoclonal antibody was generated from mice immunized with a 12 mer-peptide derived from the sequence of human MMP-9. The amino acid sequence of this peptide was: KLGLGADVAQVT (Murray et al., 1998a and 1998b).

Reactivity
This monoclonal antibody binds to human MMP-9. Reactivity in other species has not been determined. The 12-mer peptide used for the immunization differs on 4 or 5 amino acids with the sequence of MMP-9 of rat, mouse, and rabbit. Therefore it is not expected to be effective in these species as well. The antibody was tested for cross-reactivity with MMP-1, MMP-2, and MMP-3 and did not cross-react.

Technical information
The 2C3 monoclonal antibody has been tested for use in Western blotting and immunohistochemical analysis of formalin-fixed/paraffin-embedded tissues. It is suggested to use colon carcinoma as positive control. Antigen retrieval by microwave treatment (5-20 min at full power, 950 W in 0.01 M sodium citrate buffer, pH 6.0) is recommended for immunohistochemical analysis.

References
1. Murray GI, Duncan ME, O’Neil P, McKay JA, Melvin WT, Fothergill JE (1998a) Matrix metalloproteinase-1 is associated with poor prognosis in oesophageal cancer. Journal of Pathology 185: 256-261
2. Murray GI, Duncan ME, Arbuckle E, Melvin WT, Fothergill JE (1998b) Matrix metalloproteinases and their inhibitors in gastric cancer. Gut 43: 791-797