Anti-human cytokeratin no. 13 (clone 2D7)
"Binding of 2D7 monoclonal antibodies to human cytokeratin no. 13"
Hybridoma Clone Antibody Isotype Cat. No.
Clone 2D7 mouse IgG2b ARA24
Source
A mouse was immunized with cytokeratin purified from human esophagus. Antibody producing cells
were isolated after fusing the spleen cells of this mouse with murine myeloma cells by selecting the
cells that produced antibodies capable of staining human epithelia in thin sections
Specification:
The antibody does not stain non-cornifying squamous epithelium and transitional epithelium of
the bladder. In immunoblotting experiments the antibody recognizes only keratin no. 13 (54 kDa).
| | Immunohistochemistry (frozen sections) | yes |
| | Immunohistochemistry (formalin embedded tissue) | no |
| | Immunohistochemistry (antigen retrieval) | yes |
| | Flow cytometry | not tested |
| | Immunoblotting | yes |
| | ELISA | not tested |
| | RIA | not tested |
| | Cross reactivity |
| | Other cytokeratins | not detectable |
| | Vimentin | not detectable |
| | Desmin | not detectable |
| | Glial fibrillar acidic protein | not detectable |
| | Neurofilament | not detectable |
| | Mouse cytokeratin 13 | not tested |
| | Rat cytokeratin 13 | not tested |
| | Rabbit cytokeratin 13 | not tested |
| | Goat cytokeratin 13 | not tested |
Immunoglobulin (sub)class: IgG2b
Presentation: supernatant or protein A purified
Storage:
4°C = 2 months
-20°C = 1 year
Aliquoted storage at -70°C or –20°C is recommended
Reference:
1. Muijen GNP et al. (1986) Exp Cell Res 162, 97-113.
2. Muijen GNP et al. (1987) Exp Cell Res 171, 331-345.
3. Niekerk CC van et al. (1991) J Pathol 165, 145.
4. Ramaekers F (1990) Am J Pathol 136, 641.
5. Niekerk CC van et al. (1989) Int J Cancer 43, 1065.
6. Niekerk CC van et al. (1991) Am J Pathol 138, 455.
Ordering Information
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