Anti-human neurofilament (clone 2F11)
"Binding of 2F11 monoclonal antibodies to human neurofilament"
Hybridoma Clone Antibody Isotype Cat. No.
2F11 Mouse IgG1 ARA28
Source
A mouse was immunized with human neurofilament isolated from the white matter from
normal adult brain tissue. Antibody-producing cells were isolated, after fusing the spleen
cells of this mouse with murine myeloma cells, by selecting the cells that produced appropriate
antibodies as based on staining pattern in thin sections.
Specification:
The antibody does stain neurons in sections of brain and other tissues. On an immunoblot
on a crude human brain extract the antibody will only stain the 70 kDa band of
neurofilament (ref. 1)
| | Immunohistochemistry (frozen sections) | yes |
| | Immunohistochemistry (formalin embedded tissue) | yes |
| | Immunohistochemistry (antigen retieval) | yes |
| | Flow cytometry | not tested |
| | Immunoblotting | yes |
| | ELISA | not tested |
| | RIA | not tested |
| | Cross reactivity | |
| | Glial fibrillar acidic protein | not detectable |
| | Cytokeratin | not detectable |
| | Vimentin | not detectable |
| | Mouse neurofilament | not tested |
| | Rat neurofilament | not tested |
| | Rabbit neurofilament | not tested |
| | Goat neurofilament | not tested |
Immunoglobulin (sub)class: IgG1
Presentation: supernatant or protein A purified
Storage:
4°C = 2 months
-20°C = 1 year
Aliquoted storage at -70°C or –20°C is recommended
Reference:
1. Muijen GNP van et al. (1984) Am J Pathol. 116, 363-369
2. Klück P et al. (1984) Lancet (March 24th), 652-653
3. Muijen GNP van et al. (1985) Hum Pathol 16, 590-595
4. Banks-Schlegel SP et al. (1985) Cancer Res 406, 339
5. Hoefler H et al. (1985). Virch Arch 406, 339
Ordering Information
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