Monoclonal antibodies directed against rat Natural Killer cell activation structures

Hybridoma Clone    Antibody Isotype    Cat. No.
ANK61                     Mouse IgG1         ARA14

Generation
The ANK61 monoclonal antibody was generated by immunizing mice with IL2-activated cultured NK cells of Wag rats (Giezeman-Smits et al., 1997).

Reactivity
ANK61 reactivity is rat strain-independent.
The antigen recognized by ANK61 is highly expressed on freshly isolated and cultured rat NK cells. The antibodies also bind to rat aß-TCR T cells and at a low level to rat B cells. Binding to other cell types is unknown. Triggering of the antigen by ANK61 antibodies activates the lytic machinery of rat NK cells, but not of T cells.

Technical information
The ANK61 monoclonal antibody can be used in flow cytometry and for immunoprecipitation of the antigen. It can also be used for immunohistochemical analysis sections of freshly frozen tissues or formalin-fixed/paraffin-embedded tissues. Citric acid pretreatment is recommended for the latter type of tissues.

References
1. Giezeman-Smits KM, Gorter A, Nagelkerke JF, Van Vlierberghe RL, van Eendenburg J, Eggermont AM, Fleuren GJ, Kuppen PJ (1997) Characterization of three new membrane structures on rat NK cells which are involved in activation of the lytic machinery. Immunobiology 197: 429-443