Monoclonal antibodies directed against rat CD45

Hybridoma Clone    Antibody Isotype    Cat. No.
ANK74                      Mouse IgG1           ARA05
ANK12                      Mouse IgG1           ARA12

Generation
The ANK74 and ANK12 monoclonal antibodies were generated by immunizing mice with IL2-activated cultured NK cells of Wag rats.

Reactivity
These are rat strain-independent antibodies to CD45.

ANK74
This monoclonal antibody recognizes a common epitope of rat CD45, present on all rat
leucocytes.

ANK12
This monoclonal antibody is directed against an isoform of rat CD45.

Technical information
ANK74 and ANK12 bind to rat CD45. This antigen of approximately 200 kD is expressed on the cell surface of leucocytes. ANK74 and ANK12 can be used for the detection of leucocytes by immunohistochemistry in freshly frozen tissues or by flow cytometry. Both antibodies are able to modulate lysis of target cells by NK cells (Giezeman-Smits et al., 1999).

References
Giezeman-Smits KM, Gorter A, Van Vlierberghe RL, Eendenburg JD, Eggermont AM, Fleuren GJ, Kuppen PJ (1999) The regulatory role of CD45 on rat NK cells in target cell lysis. J Immunol 163: 71-76