Anti-human cytokeratin (clone 80)
"Binding of Clone 80 monoclonal antibodies to human cytokeratin"
Hybridoma Clone Antibody Isotype Cat. No.
Clone 80 Mouse IgG1 ARA19
Source
A mouse was immunized with callus cytokeratins isolated from fresh human skin tissue.
Antibody producing cells were isolated - after fusing the spleen cells of this mouse with
murine myeloma cells by selecting the cells that produced antibody capable of staining
human epithelia in thin sections.
Specification:
The antibody does stain all types of keratin containing (that is epithelial) cells in frozen
sections of various tissues, with the exception of myo-epithelial cells and the podocytes of
the glomeruli.
| | Immunohistochemistry (frozen sections) | yes |
| | Immunohistochemistry (formalin embedded tissue) | no |
| | Immunohistochemistry (antigen retrieval) | yes |
| | Flow cytometry | yes |
| | Immunoblotting | yes |
| | ELISA | not tested |
| | RIA | not tested |
|
| | Cross reactivity |
| | | Neurofilament | not detectable |
| | Vimentin | not detectable |
| | Glial fibrillar acidic protein | not detectable |
| | Mouse cytokeratins | yes |
| | Rat cytokeratins | yes |
| | Rabbit cytokeratins | not tested |
| | Goat cytokeratins | not tested |
Immunoglobulin (sub)class: IgG1
Presentation: supernatant
Storage:
4°C = 2 months
-20°C = 1 year
Aliquoted storage at -70°C or –20°C is recommended
Reference:
1. Muijen GNP van (1984) Am J Pathol 114, 9.
2. Muijen GNP van (1984) Am J. Pathol 116, 363.
3. Muijen GNP van (1985) Hum Pathol 16, 590.
4. Corver WE et al. (2000) Cytometry 41, 73-80.
5. Broek, JCM van et al. (2000) Appl. Immunohis. & Mol. Morph. 8, 316-321.
Ordering Information
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