Monoclonal antibodies directed against laminin

Hybridoma Clone    Antibody Isotype    Cat. No.
MEC3                     Rat IgM                 ARA15
MEC5                     Rat IgG1                ARA16

Generation
MEC3 and MEC5 are auto-antibodies derived from DZB rats that had developed membranous glomerulopathy upon exposure to mercuric chloride (Aten et al. 1995).

Reactivity
Both rat monoclonal antibodies are directed against the P1 fragment of laminin. They are species-independent as it has been shown that they bind to laminin of rat, mouse and humans (Aten et al., 1995).

Technical information
MEC3 and MEC5 monoclonal antibodies are especially useful for immunohistochemical detection of laminin in extracellular matrix or basement membranes in freshly frozen tissue sections of rat, mouse or humans. For MEC5 it is recommended to pretreat the sections with pepsin.

References
Aten J, Veninga A, Coers W, Sonnenberg A, Timpl R, Claessen N, Van Eendenburg JD, de Heer E, Weening JJ (1995) Autoantibodies to the laminin P1 fragment in HgCl2-induced membranous glomerulopathy. Am J Pathol 146: 1467-1480