Anti-human cytokeratin no. 8 (clone M20)
"Binding of M20 monoclonal antibodies to human cytokeratin no. 8"
Hybridoma Clone Antibody Isotype Cat. No.
M20-2B Mouse IgG2b ARA22
Source
A mouse was immunized with cytokeratin isolated from a human breast carcinoma cell line (MCF-7).
Antibody producing cells were isolated after fusing the spleen cells of this mouse with murine myeloma
cells by selecting the cells that produced antibodies capable of staining human simple epithelia in
thin sections.
Specification:
The antibody stains almost all epithelial cells. In immunoblotting experiments the antibody was shown to
recognize cytokeratin no. 8 (52 kDa).
| | Immunohistochemistry (frozen sections) | yes |
| | Immunohistochemistry (formalin embedded tissue) | no |
| | Immunohistochemistry (antigen retrieval) | yes |
| | Flow cytometry | yes |
| | Immunoblotting | yes |
| | ELISA | not tested |
| | RIA | not tested |
|
| | Cross reactivity |
| | | Other cytokeratins | not detectable |
| | Vimentin | not detectable |
| | Desmin | not detectable |
| | Glial fibrillar acidic protein | not detectable |
| | Neurofilament | not detectable |
|
| | Mouse cytokeratin 8 | not tested |
| | Rat cytokeratin 8 | not tested |
| | Rabbit cytokeratin 8 | not tested |
| | Goat cytokeratin 8 | not tested |
Immunoglobulin (sub)class: IgG2b
Presentation: supernatant or protein A purified
Storage:
4°C = 2 months
-20°C = 1 year
Aliquoted storage at -70°C or –20°C is recommended
Reference:
1. Muijen GNP et al. (1987) Lab Invest 57, 359-369.
2. Muijen GNP et al. (1987) Exp Cell Res 171, 331-345.
3. Dorst EBL et al. (1998) J Clin Pathol 51, 679-684.
4. Corver WE et al. (1995) Cytometry 19, 267-272.
Ordering Information
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