Monoclonal antibodies directed against rat tumor antigens of epithelial origin

          
           "Binding of MG2 monoclonal antibodies to rat colorectal
           metastases in freshly frozen rat liver tissue"

Hybridoma Clone    Antibody Isotype    Cat. No.
MG1                       Mouse IgG2a        ARA02
MG2                       Mouse IgG2a        ARA10

Generation
MG1 and MG2 have been raised by immunization of mice with rat colon adenocarcinoma cells (CC531). Hybridomas were selected for producing antibodies reacting with the CC531 cell line and with minimal cross reactivity to normal tissues (Hagenaars et al., 2001). MG1 and MG2 belong to a second generation of antibodies directed against tumors of epithelial origin that were strictly selected for minimal cross reactivity with other tissues.

Reactivity
MG1 and MG2 are rat strain-independent markers for tumor cells of epithelial origin, like in colon, breast, or lung cancer, etc. When injected in colon tumor-bearing rats, MG1 and MG2 localized to tumor cells (Hagenaars et al., 2001). MG1 and MG2 can be used for immunohistochemical staining of tumor cells in frozen tissue sections as well as formalin-fixed/paraffin-embedded tissue. MG1 and MG2 are also suitable to be used in flow cytometry, Western blotting, immunoprecipitation, and ELISA.

Technical information

MG1
This monoclonal antibody recognizes a cell surface structure of about 80 kD expressed by rat tumor cells of epithelial origin. It is useful for in vivo applications as it has no detectable binding to other cell types then tumor cells when injected in rats. MG1 is also useful for immunohistochemical detection of tumor cells. In immunohistochemical stainings, there is some background in thymus and lymph nodes. Citric acid or EDTA pretreatment is recommended for formalin-fixed/paraffin-embedded tissue sections.

MG2
This monoclonal antibody has the same characteristics as MG1.

References
1. Hagenaars M, Ensink NG, Basse P, Hokland M, Nannmark U, Eggermont AM, Van De Velde CJ, Fleuren GJ, Kuppen PJ (2000) The microscopic anatomy of experimental rat CC531 colon tumour metastases: consequences for immunotherapy? Clin Exp Metastasis 18: 189-196.
2. Hagenaars M, Ensink NG, Van Eendenburg JD, Van Vlierberghe RL, Eggermont AM, Van De Velde CJ, Fleuren GJ, Kuppen PJ (2001) The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma. Clin Exp Metastasis, 18: 281-289.