Monoclonal antibodies directed against rat tumor antigens of epithelial origin

Hybridoma Clone    Antibody Isotype    Cat. No.
MG3                       Mouse IgG3          ARA11


Generation
MG3 has been raised by immunization of mice with rat colon adenocarcinoma cells (CC531). Hybridomas were selected for producing antibodies reacting with the CC531 cell line and with minimal cross reactivity to normal tissues (Hagenaars et al., 2001). MG3 belongs to a second generation of such antibodies that were strictly selected for minimal cross reactivity with other tissues.

Reactivity
MG3 is a rat strain-dependent marker for tumor cells of epithelial origin, like in colon, breast, or lung cancer, etc. When injected in colon tumor-bearing rats, MG3 did not localize to tumor cells (Hagenaars et al., 2001). MG3 is able to activate the rat complement system. MG3 can be used for immunohistochemical staining of tumor cells in frozen tissue sections as well as formalin-fixed/paraffin-embedded tissue. MG3 is also suitable to be used in flow cytometry, Western blotting, immunoprecipitation, and ELISA.

Technical information
The antigen detected by this monoclonal antibody has not been characterized. The antigen is expressed on the cell surface of rat tumor cells of epithelial origin. When bound to tumor cells the antibody activates the rat complement system.

References
1. Hagenaars M, Ensink NG, Basse P, Hokland M, Nannmark U, Eggermont AM, Van De Velde CJ, Fleuren GJ, Kuppen PJ (2000) The microscopic anatomy of experimental rat CC531 colon tumour metastases: consequences for immunotherapy? Clin Exp Metastasis 18: 189-196.
2. Hagenaars M, Ensink NG, Van Eendenburg JD, Van Vlierberghe RL, Eggermont AM, Van De Velde CJ, Fleuren GJ, Kuppen PJ (2001) The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma. Clin Exp Metastasis, 18: 281-289.