Anti-human melanoma (clone PAL-M1)
"Binding of PAL-M1 monoclonal antibodies to human melanoma"
Hybridoma Clone Antibody Isotype Cat. No.
PAL-M1 Mouse IgG1 ARA35
Source
A mouse was immunized with tissue derived from human melanoma metastases.
After fusing the spleen cells from this mouse with myeloma cells, antibody-producing cells were
screened on thin sections of various human tissues. Clone PAL-M1 was identified that produced
antibodies staining malignant melanomas.
Specification:
Antibody PAL-M1 stains the majority of primary melanomas and the large majority of metastases
and is directed against the transferrin receptor (CD71). Local staining of dysplastic nevocellular
nevi may be observed. Common nevocellular nevi rarely stain.
| | Immunohistochemistry (frozen sections) | yes |
| | Immunohistochemistry (formalin embedded tissue) | no |
| | Immunohistochemistry (antigen retrieval) | no |
| | Flow cytometry | yes |
| | Immunoblotting | yes |
| | ELISA | not tested |
| | RIA | not tested |
| | Cross reactivity: |
| | Skin carcinoma | not detectable |
| | Dendritic cells in inflammatory infiltrates | present |
| | Sebaceous glands | present |
| | Non-cutaneous tumor | present |
| | Mouse melanoma | not tested |
| | Rat melanoma | not tested |
| | Rabbit melanoma | not tested |
| | Goat melanoma | not tested |
Immunoglobulin (sub)class: IgG1
Presentation: supernatant
Storage:
4°C = 2 months
-20°C = 1 year
Aliquoted storage at -70°C or –20°C is recommended
Reference:
1. Ruiter DJ et al., (1985) J Invest Dermatol 85, 4-8.
2. Muijen G van et al. (1990) J Invest Dermatol 95, 65-69.
3. Corver WE et al. (1994) Cytometry 19, 267-272.
Ordering Information
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