Anti-human vimentin (clone V9)
"Binding of V9 monoclonal antibodies to human vimentin"
Hybridoma Clone Antibody Isotype Cat. No.
V9-2b Mouse IgG2b ARA27
Source
A mouse was immunized with vimentin isolated from cow lens.
Antibody-producing cells were isolated after fusing the spleen cells of the mouse with
murine myeloma cells by selecting the cells that produced antibodies capable of staining
mesenchymal cells in thin sections of human colon.
Specification:
The antibody does stain all cells that contain vimentin. Only frozen section can be used.
The antibody only stains the 57kDa vimentin band in immunoblots performed on a lysate of
human lymphocytes.
| | Immunohistochemistry (frozen sections) | yes |
| | Immunohistochemistry (formalin embedded tissue) | no |
| | Immunohistochemistry (antigen retieval) | yes |
| | Flow cytometry | yes |
| | Immunoblotting | yes |
| | ELISA | not tested |
| | RIA | not tested |
| | Cross reactivity: |
| | Neurofilament | not detected |
| | Glial fibrillar acidic protein | not detected |
| | Desmin | not detected |
| | Cytokeratin | not detected |
| | Mouse vimentin | not tested |
| | Rat vimentin | not tested |
| | Rabbit vimentin | not tested |
| | Goat vimentin | not tested |
Immunoglobulin (sub)class: IgG2b
Presentation: supernatant or protein A purified
Storage:
4°C = 2 months
-20°C = 1 year
Aliquoted storage at -70°C or –20°C is recommended
Reference:
1. Muijen GNP van (1984) Am J Pathol 116, 363-369.
2. Muijen GNP van (1985) Hum Pathol 16, 590-595.
3. Muijen GNP van (1987) Lab Invest 57, 359-369.
4. Debus E et al. (1984) Eur J Cell Biol 34, 137-143.
5. Muijen G van (1986) Exp Cell Res 162; 97-113
6. Corver WE et al. (1994) Cytometry 15, 117-128
7. Broek JCM van den et al. Appl Immunohis & Mol Morphology 8, 316-321, 2000
Ordering Information
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