Monoclonal antibodies directed against rat tumor antigens of epithelial origin

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                "Binding of CC52 monoclonal antibodies to rat colorectal
                metastases in paraffin-embedded liver tissue"

Hybridoma Clone    Antibody Isotype    Cat. No.
CC52                        Mouse IgG1            ARA01

Generation
  These monoclonal antibodies have been raised by immunization  of mice  with rat 
  colon adenocarcinoma cells (CC531). Hybridomas were  selected for producing 
  antibodies reacting with the CC531 cell line and with minimal cross reactivity 
  to normal tissues (Hagenaars et al., 2001). CC52 belongs to a first generation of 
  such antibodies and is very useful to detect tumor cells by immunohistochemistry.
Reactivity 
  CC52 is a rat strain-independent marker for tumor cells of epithelial origin, like in colon, breast, 
  or lung cancer, etc. When injected in colon tumor-bearing rats, CC52 localized to tumor cells 
  (Hagenaars et al., 2001). CC52 can be used for immunohistochemical staining of tumor cells 
  in frozen tissue sections as well as formalin-fixed/paraffin-embedded tissue. CC52 is also  
  suitable to be used in flow cytometry, Western blotting, immunoprecipitation, and ELISA.
Technical information 
  The monoclonal antibody binds to two proteins, 120 kD and 130 kD, expressed by rat tumor 
  cells of epithelial origin. CC52 has been used in many studies in a rat model of colorectal 
  cancer  (e.g. Hagenaars et al., 2000). In immunohistochemical stainings, there is some 
  background  in intestine epithelial tissue and bile ducts. Citric acid pretreatment is 
  recommended for formalin-fixed/paraffin-embedded tissue sections. The antigens detected 
  by CC52 are also  expressed by IL-2-activated cultured NK cells and T cells and by bone 
  marrow cells. CC52  can also be used in Western blotting. In addition, there is a bispecific 
  antibody available (R73xCC52, ARA17) that consists for one half of this antibody and the 
  other half of an antibody directed against the rat T cell receptor. This bispecific antibody is 
  able to induce T cell to lyse tumor cells.
References 
  Hagenaars M, Ensink NG, Basse P, Hokland M, Nannmark U, Eggermont AM, Van De Velde CJ, 
  Fleuren GJ, Kuppen PJ (2000) The microscopic anatomy of experimental rat CC531 colon 
  tumour metastases: consequences for immunotherapy? Clin Exp Metastasis 18: 189-196

  Hagenaars M, Ensink NG, Van Eendenburg JD, Van Vlierberghe RL, Eggermont AM, Van 
  De Velde CJ, Fleuren GJ, Kuppen PJ (2001) The development of novel mouse monoclonal 
  antibodies against the CC531 rat colon adenocarcinoma. Clin Exp Metastasis, 18: 281-289
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